Fig. 3
From: Process engineering of pH tolerant Ustilago cynodontis for efficient itaconic acid production
Controlled high-density fed-batch fermentation of U. cynodontis NBRC 9727 ∆fuz7r ∆cyp3r PetefmttA Pria1ria1; a glucose concentration; b OD600 (circles) and ammonium (squares) concentration; c itaconate (diamonds, red) and erythritol (circles) concentration and d CO2 off-gas concentration during fermentation in a bioreactor containing batch medium with 50 g L−1 glucose, 0.8 g L−1 NH4Cl with a natural pH shift from 6 to 3.6 (filled symbols/continuous grey line) or a constant pH of 3.6 (empty symbols/continuous black line) controlled with NaOH. Arrows indicate addition of 35 mL of 50% glucose. Error bars indicate the standard error of the mean (n = 3)