Table 7 Sequences of primers used in this study for qRT-PCR
Target gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | GenBank accession number |
|---|---|---|---|
TNFα | CCCTTATCATTATGGCCCTT | TCCGAGTACCGCCATATCCT | FJ654645.1 |
IL-1β | TACCTGTCGTGCCAACAGGAA | TGATGTACCAGTTGGGGAA | AJ295836.2 |
TGFβ1 | CTGCAGGACTGGCTCAAAGG | CATGGTCAGGATGTATGGTGGT | KU238187 |
MUC-2 | GTTGGTGCAGCCGCATAG | CACTGGACGCTGGGAATG | KU238186 |
ZO-1 transcript variant 1 | AGAGAACCTGTCACTGATAGATGC | CTGTCGGAATTGTTGCCTGATG | KU238184 |
Occludin | ACTGGCATTCTTCATCGC | GGTACAGATTCTGGCACATC | KU238182 |
Tricellulin | GCCTACATCCACAAAGACAACG | TCATTCCCAGCACTAATACAATCAC | KU238183 |
β-Actin | GCTGTCTTCCCTTCTATCGTCG | TCCATGTCATCCCAGTTGGTC | AY008305.1 |