Fig. 5
The intestinal morphology, integrity, and inflammation status of turbot fed experimental diets. a Representative histomorphological images from hematoxylin and eosin-stained sections and the villi height analysis of the distal intestine of turbot. b Effects of experimental diets on intestinal integrity marker gene expressions of turbot. c Effects of experimental diets on intestinal inflammation marker gene expressions of turbot. The value of FM group was normalized to 1.0 and the rest groups were expressed as relative expression levels to the FM group. Values show mean ± standard error (n = 6) and different letters (a, b, c) on top represent statistically significant results (P < 0.05) based on one-way analysis of variance (ANOVA) by the software SPSS 19.0. R villi height/lumen diameter, arbitrary units, TNF-α tumor necrosis factor-α, IL-1β interleukin-1β, TGF-β1 transforming growth factor-β1, MUC-2 mucin 2, ZO-1 zonula occluden-1