Table 1 Strains and plasmids used in this work
Strain/plasmids | Relevant characteristics | Source or reference |
|---|---|---|
Strains | ||
 S. erythraea NRRL2338 | Used as parental strain, wild type | DSM 40517 |
 E. coli DH5α | F−Ø80d lacZΔM (lacZYA -argF)U169 deoR | Invitrogen |
 E. coli BL21(DE3)-6965 | The strain for expression of PhoP | This report |
 E. coli BL21(DE3) | F′ompTr-Bm-B(λDE3) | This report |
 S. erythraea OphoP | The strain for over-expression of phoP, NRRL2338 integrated with pIB139-6965 | This report |
 S. erythraea ∆glnR | NRRL2338 glnR::tsr (glnR null mutant) | This report |
 S. erythraea CglnR | glnR complementary strain, ∆glnR carrying pIB-glnR | This report |
 S. erythraea ∆glnR:  OphoP | The strain for over-expression of phoP, ∆glnR integrated with pIB139-6965 | This report |
Plasmids | ||
 pET28a | Expression vector | Novagen |
 P6965 | pET28a derivative carrying phoP of S. erythraea | This report |
 pIB139 | Escherichia coli–S. erythraea integrative shuttle vector containing a strong constitutive | [20] |
 pIB139-6965 | pIB139 carrying an extra phoP for the gene overexpression | This report |
 pIB-glnR | pIB139 carrying an extra glnR for the gene overexpression | This report |