Fig. 6
Plasmid-based expression of a second ABA gene in strain SABA3. The gene(s) expressed from the plasmids are listed in brackets below the strains. All genes were expressed from p416 GPD controlled by PTDH3 or PTEF1 and PTDH3 in case of S3S12. The control strain S3S0 carries an empty plasmid. Measurements were taken after 48 h of cultivation in minimal medium. Average and standard deviation were calculated from three independent biological replicates. a ABA titre in culture supernatant and OD600. Grey lines below the graph show statistical significance compared to the control strain S3S0 (*significant, p < 0.05; **highly significant, p < 0.01). b Ratio of ion counts for ABA and MZ233. The ratios of S3S1, S3S3, S3S4 and S3S12 were significantly different compared to the control strain S3S0 (p < 0.05), while S3S2 showed a highly significant difference (p < 0.01)