Fig. 3

ABA titre in supernatant and OD₆₀₀ of strains based on 5D-tHMG1. Strains were cultivated for 48 h in minimal medium supplemented with uracil. Average ABA titres and maximum OD₆₀₀ were calculated from three independent biological replicates. Tables below the graphs list heterologous genes present in the different strains or in the case of NCP1 a native overexpressed gene. a Effect of bcaba3, bcaba5 and bcceP450 on ABA titres. No ABA was detected for TABA4. The strains were cultivated in shake flasks. b Effect of CPR overexpression on ABA titres. TABA3 expresses the B. cinerea CPR gene bccpr1, TABA5 overexpresses the native S. cerevisiae CPR gene NCP1 and TABA6 does not overexpress a CPR. The strains were cultivated in deep-well plates