Table 1 List of strains used in this study
From: Efficient Cas9-based genome editing of Rhodobacter sphaeroides for metabolic engineering
Strain | Description | Source of reference |
|---|---|---|
E. coli | ||
DH5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17Strain for assembly and plasmid amplification | Laboratory stock |
S17-1 | Host strain for transconjugation, thi pro recA hsdR [RP4-2Tc::Mu-Km::Tn7] Tpr Smr | Laboratory stock |
R. sphaeroides | ||
265-9c | Wild type | Derivative of ATCC 35053 |
Δupp | 265-9c Δupp | This study |
uppKI | 265-9c KI(ATG->GTG)upp | This study |
ΔphaB | 265-9c ΔphaB | This study |
ΔphbB | 265-9c ΔphbB | This study |
ΔphaB_ΔphbB | 265-9c ΔphaB-ΔphbB | This study |