Fig. 7
From: Synthetic control devices for gene regulation in Penicillium chrysogenum
Schematic representation of CRISPR/Cas9 mediated co-transformation of the synthetic control device into the penicillin cluster. The marker-free donor DNA carried QUAS elements with flanking regions for homologous recombination at the pcbAB/pcbC locus. The amdS marker carrying integration cassette delivers the STF and a 5xQUAS element upstream of the core promoter of penDE gene