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Table 2 Bacterial strains and plasmids utilized in this study

From: Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system

ItemGenotype of phenotypeReferences
Strains
 E. coli DH5αCloning host; F -φ80lacZ∆M15endA1 recA1 endA1 hsdR17 (rK-mK +)supE44 thi-1 gyrA 96 relA1(lacZYAargF)U169 deoR λ[39]
 L. lactis N8Nisin Z producing strain[40]
 L. lactis MG1363L. lactis subsp. cremoris, plasmid-free derivative of NCDO712[41]
 L. lactis NZ9000 (9k)MG1363 pepN::nisRK, the original strain[42]
 L. lactis 9k-3Three fragments (about 50 kb) deletion in L. lactis 9K[14]
 L. lactis 9k-4AThe L4A (about 10 kb) deletion in L. lactis 9k-3This work
 L. lactis 9k-5AThe L5A (about 21.5 kb) deletion in L. lactis 9k-4AThis work
Plasmids
 pNZ5319Cmr, Emr, the original vector[20]
 pNZ5319Δ L4ACmr, Emr, L4-A knock-out vectorThis work
 pNZ5417Cmr, Derivative of pNZ5319; Δery; containing PnisZ::lacZThis work
 pNZ5417Δ L4ACmr, L4-A knock-out vectorThis work
 pNZ5417Δ L5ACmr, L5-A knock-out vectorThis work
 pNZTS-CreEmr, cre gene cloned at the EcoRI and HindIII sites[19]
 pNZ8048Cmr[42]