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Table 1 The loss efficiency of recycling gRNA expression plasmid P426-CL

From: High production of valencene in Saccharomyces cerevisiae through metabolic engineering

Dilution factor (D)

Colony number

Loss efficiency (%)

Plate 1

Plate 2

Plate 3

Average

103

12

15

47

25

99.98

104

1

3

2

2

99.98

105

0

0

1

0

100

  1. The plasmid loss efficiency was calculated based on following equation \( {\text{Plasmid loss efficiency}} = \frac{{A_{1} - D \times \left( {A_{2} \div 0.2} \right)}}{{A_{1} }} \)
  2. A1 = The density of yeast solution, CFU/mL, A2 = The colony number in the solid plate, D = The dilution factor. Each dilution factor was done in triplicate
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Microbial Cell Factories

ISSN: 1475-2859

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