Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Extracellular production of the engineered thermostable protease pernisine from Aeropyrum pernix K1 in Streptomyces rimosus

Fig. 5

Analysis of codon-optimised pernisine (plasmid construct pVFPER4) and srT-pernisine (plasmid construct pVFPER5) from culture supernatants from S. rimosus. Proteins from culture supernatant were separated by SDS-PAGE (a) and zymography (b). The proteins were transferred onto nitrocellulose membranes and His6-tagged pernisine was detected using anti-His6 tag antibodies (c). Lanes 0, protein MW markers; lanes 1, srT-pernisineCO; lanes 2, negative control; lanes 3, pernisineCO. d Additionally, the amounts of secreted pernisineCO (left) and srT-pernisineCO (right) were estimated using dot blot assays

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us