Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

Table 5 Plasmids and strains used in this study

Name	Parental plasmids	Description	Reference
Plasmids
pCfB258		Integrative plasmid, pX-4-loxP, SpHIS5, P_TEF1-T_ADH1, P_GKp1-T_CYC1	[21]
pCfB390		Integrative plasmid, pXI-3-loxP, KlURA3, P_TEF1-T_ADH1, P_PGK1-T_CYC1	[21] ]
pCfB258		Integrative plasmid, pX-4-loxP, SpHIS5, P_TEF1-T_ADH1, P_PGK1-T_CYC1	[21]
pCfB0826		Integrative plasmid, pX-4-LoxP, SpHiS5, P_TEF1-ScAro7^fbr-T_ADH1, P_PGK1-ScAro4^fbr-T_CYC1	[44]
pCfB1964		Integrative plasmid, pX-2-loxP, KlURA3, P_TEF1-Fj_TAL-T_ADH1	[44]
pCfB2747	pCfB3034	Integrative plasmid, X-3, P_PGK1- EcaroL, KlLEU2	[44]
pCfB3524	pCfB390	Integrative plasmid, pXI-3-loxP, KlURA3, BB380 (Fj_TAL<-), BB010 (<-P_TEF1-P_PGK1->), BB0501 (EcaroL->)	This study

Name	Description	Reference
Strains
ST2488	CEN.PK 113-3C/pRS314-X123 (P_TDH3-PsXYL1, P_TDH3-PsXYL2, P_TDH3-PsXYL3 TRP1) TRP1 ura3Δ his3Δ leu2Δ	[56]
ST4274	CMB.GS010Δaro10::Leu27/pRS314-X123 (P_TDH3-PsXYL1, P_TDH3-PsXYL2, P_TDH3-PsXYL3 TRP1) Δpdc5::Kanmx TAL AroL::URA3 Aro7-Aro4::His5	This study

ISSN: 1475-2859