Redirecting photosynthetic electron flux in the cyanobacterium Synechocystis sp. PCC 6803 by the deletion of flavodiiron protein Flv3

Table 1 The sucrose-producing Synechocystis strains generated and compared in this study

Strain ID	Genetic background	OE target(s)	Expression plasmid
S01	Wild-type	CscB	pDF-lac2-cscB-CmR
S01:Δflv3	Δflv3	CscB	pDF-lac2-cscB-CmR
S01:compl	Δflv3	CscB + Flv3	pDF-lac2-cscB-flv3-CmR
S02	ΔggpS	CscB + SPS	pDF-lac2-cscB-sps-CmR
S02:Δflv3	Δflv3 + ΔggpS	CscB + SPS	pDF-lac2-cscB-sps-CmR

The enzymes targeted for inactivation and over-expression are Flv3 (flavodiiron protein 3 from Synechocystis), GGPS (glucosylglycerolphosphate synthase from Synechocystis), CscB (sucrose permease from E. coli), and SPS (sucrose phosphate synthase from Synechocystis)

ISSN: 1475-2859