Table 1 Effect of additives on Lp_0179 and Lp_2757 α-amylase activity
Additives (1 mM) | Relative activity (%) | |
|---|---|---|
Lp_0179 | Lp_2757 | |
Control | 100 | 100 |
KCl | 93 | 90 |
HgCl2 | 6 | 2 |
CaCl2 | 98 | 81 |
MgCl2 | 96 | 58 |
ZnCl2 | 92 | 69 |
CuCl2 | 85 | 5 |
NiCl2 | 87 | 17 |
MnCl2 | 98 | 89 |
FeCl2 | 102 | 108 |
Tween 20 | 95 | 110 |
Tween 80 | 95 | 117 |
Triton X-100 | 96 | 120 |
SDS | 86 | 59 |
Urea | 100 | 95 |
DMSO | 99 | 104 |
Cysteine | 101 | 92 |
β-mercaptoethanol | 99 | 107 |
EDTA | 99 | 94 |
PMSF | 68 | 134 |
DEPC | 94 | 102 |