Fig. 8

Metabolic characterization of the MEP pathway in E. coli expressing isoprene synthase and isopentenyl pyrophosphate isomerase from a plasmid. The intracellular metabolite concentrations, secretion rate of metabolites and isoprene production rate are plotted against dxs expression. The expression strength of dxs was modulated by randomization of its ribosome-binding site. DXP 1-deoxy-d-xylulose 5-phosphate, MEP 2-C-methyl-d-erythritol 4-phosphate, MEcPP 2-C-methyl-d-erythritol 2,4-cyclopyrophosphate, ME-CDP 4-diphosphocytidyl-2-C-methylerythritol, MEP-CDP 4-diphosphocytidyl-2-C-methyl-d-erythritol 2-phosphate, HMBPP 4-hydroxy-3-methyl-but-2-enyl pyrophosphate, IPP isopentenyl pyrophosphate, DMAPP dimethylallyl pyrophosphate, GAP glyceraldehyde 3-phosphate, Dxs DXP synthase, Dxr DXP reductoisomerase, IspD MEP cytidylyltransferase, IspE ME-CDP kinase, IspF MEcPP synthase, IspG HMBPP synthase, IspH HMBPP reductase, Idi isopentenyl diphosphate isomerase, IspS isoprene synthase