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Table 1 Strains and plasmids used in this study

From: High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium

Strain or plasmid Relevant phenotype or genotype Reference
Strain
 Halomonas hydrothermalis Y2 Halotolerant alkaliphilic strain [26]
 E. coli DH5α Competent cell for cloning  
 E. coli S17-1 Competent cell for gene knockout  
 Y2/ΔectD Strain Y2 with ectD deficient This study
 Y2/ΔectD/ΔdoeA Strain Y2 with ectD and doeA deficient This study
 Y2/ΔectD/ΔdoeA/ΔnhaD2 Strain Y2 with ectD, doeA, and nhaD2 deficient This study
 Y2/ΔectD/ΔdoeA/Δmrp Strain Y2 with ectD, doeA, and mrp deficient This study
 Y2/ΔectD/ΔdoeA/ΔnhaD2/Δmrp Strain Y2 with ectD, doeA, nhaD2, and mrp deficient This study
Plasmid
 pEASYBlunt Cloning vector TransGen Biotech
 pK18mobSacB Kmr mobsacB  
 pK18mobSacBectD pK18mobSacB with ΔectD This study
 pK18mobSacBectD/ΔdoeA pK18mobSacB with ΔectD and ΔdoeA This study
 pK18mobSacBectD/ΔdoeA/ΔnhaD2 pK18mobSacB with ΔectD, ΔdoeA, ΔnhaD2 This study
 pK18mobSacBectD/ΔdoeA/Δmrp pK18mobSacB with ΔectD, ΔdoeA, Δmrp This study
 pK18mobSacBectD/ΔdoeA/ΔnhaD2/Δmrp pK18mobSacB with ΔectD, ΔdoeA, ΔnhaD2, Δmrp, This study