Table 1 Strains and plasmids used in this study
From: High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium
Strain or plasmid | Relevant phenotype or genotype | Reference |
|---|---|---|
Strain | ||
 Halomonas hydrothermalis Y2 | Halotolerant alkaliphilic strain | [26] |
 E. coli DH5α | Competent cell for cloning |  |
 E. coli S17-1 | Competent cell for gene knockout |  |
 Y2/ΔectD | Strain Y2 with ectD deficient | This study |
 Y2/ΔectD/ΔdoeA | Strain Y2 with ectD and doeA deficient | This study |
 Y2/ΔectD/ΔdoeA/ΔnhaD2 | Strain Y2 with ectD, doeA, and nhaD2 deficient | This study |
 Y2/ΔectD/ΔdoeA/Δmrp | Strain Y2 with ectD, doeA, and mrp deficient | This study |
 Y2/ΔectD/ΔdoeA/ΔnhaD2/Δmrp | Strain Y2 with ectD, doeA, nhaD2, and mrp deficient | This study |
Plasmid | ||
 pEASYBlunt | Cloning vector | TransGen Biotech |
 pK18mobSacB | Kmr mobsacB |  |
 pK18mobSacB/ΔectD | pK18mobSacB with ΔectD | This study |
 pK18mobSacB/ΔectD/ΔdoeA | pK18mobSacB with ΔectD and ΔdoeA | This study |
 pK18mobSacB/ΔectD/ΔdoeA/ΔnhaD2 | pK18mobSacB with ΔectD, ΔdoeA, ΔnhaD2 | This study |
 pK18mobSacB/ΔectD/ΔdoeA/Δmrp | pK18mobSacB with ΔectD, ΔdoeA, Δmrp | This study |
 pK18mobSacB/ΔectD/ΔdoeA/ΔnhaD2/Δmrp | pK18mobSacB with ΔectD, ΔdoeA, ΔnhaD2, Δmrp, | This study |