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Table 1 Strains and plasmid used in this study

From: Metabolic perturbations in mutants of glucose transporters and their applications in metabolite production in Escherichia coli

 

Description

Reference

Strain

 ST1

Escherichia coli W KCTC1039

Korean collection for type cultures

 ST2

ST1 ptsG::FRT

This study

 ST8

ST2 mglB::FRT malE::FRT galP::FRT

This study

 STE1

ST1 harboring pEGFP

This study

 STE2

ST2 harboring pEGFP

This study

 STE8

ST8 harboring pEGFP

This study

 STG1

ST1 gabT::FRT KanR FRT harboring pGABA

This study

 STG2

ST2 gabT::FRT KanR FRT harboring pGABA

This study

 STG8

ST8 gabT::FRT KanR FRT harboring pGABA

This study

 STL1

ST1 harboring pACYC DXS and pCDF Idi IspA CrtEBI

This study

 STL2

ST2 harboring pACYC DXS and pCDF Idi IspA CrtEBI

This study

 STL8

ST8 harboring pACYC DXS and pCDF Idi IspA CrtEBI

This study

Plasmid

 pKD46

Red recombinase expression plasmid, repA, pSC101ori, PBAD, gam, beta alpha, recA, AmpR

Datsenko and Wanner [22]

 707FLP

Flippase expression plasmid, pSC101ori, repA, cl578, FLPe, TetR

Generidge

 pZA31 MCS

CmR, p15A ori, PLtetO, rrnB T1

Expressys

 pEGFP

EGFP expression in pZA31 MCS

This study

 pGABA

GltB and GltD from E. coli, GadBmut (Glu89Gln/Δ452–466) and GadCmut(1–470) from C. glutamicum expression in pZA31 MCS

This study

 pACYC DXS

PBBa J23100::Dxs::TBBa B1005 in pACYC Duet-1

Jung et al. [69]

 pCDF Idi IspA CrtEBI

PBBa J23100::idi::TBBa B1005, PBBa J23100::ispA::TBBa B1005, PBBa_J23100::crtE-crtB-crtI::TBBa B1005 in pCDF Duet1

Jung et al. [69]