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Table 1 Experimental factors and CCD levels in the optimization of the production of ScAGal by BJ3505/YEpMEL1His

From: Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides

Real values Coded valuesa
− 2 − 1 0 1 2
Aeration; A (Vc/Ve)b 0.5 (50/100) 0.6 (40/100) 0.7 (30/100) 0.8 (20/100) 0.9 (10/100)
Glucose (%); G 0.5 1.0 1.5 2.0 2.5
pH; P 4 5 6 7 8
Time (h); T 48 72 96 120 144
  1. axi= (Xi−X0)/∆Xi; where xi and Xi are the coded and real values of the independent variable i, X0 is the real value of the independent variable i at the central point, and ∆Xi is the step change value. bAeration = 1 − (Vc/Ve); volume ratio of culture medium in mL (Vc) in a 100 mL Erlenmeyer flask (Ve)