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Table 1 Experimental factors and CCD levels in the optimization of the production of ScAGal by BJ3505/YEpMEL1His

From: Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides

Real values

Coded valuesa

− 2

− 1

0

1

2

Aeration; A (Vc/Ve)b

0.5 (50/100)

0.6 (40/100)

0.7 (30/100)

0.8 (20/100)

0.9 (10/100)

Glucose (%); G

0.5

1.0

1.5

2.0

2.5

pH; P

4

5

6

7

8

Time (h); T

48

72

96

120

144

  1. axi= (Xi−X0)/∆Xi; where xi and Xi are the coded and real values of the independent variable i, X0 is the real value of the independent variable i at the central point, and ∆Xi is the step change value. bAeration = 1 − (Vc/Ve); volume ratio of culture medium in mL (Vc) in a 100 mL Erlenmeyer flask (Ve)
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Microbial Cell Factories

ISSN: 1475-2859

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