Fig. 3

Effect of acid-neutral proteases on the activity of ScAGal. Each protease (2 mg/mL) dissolved in its reaction buffer was incubated at 37 °C in 1 U/mL ScAGal (a) and deglycosylated ScAGal (b), and the activity was measured at different times of digestion. The negative control was the enzyme acting in the absence of protease, and positive control was β-galactosidase E. coli (βGal) under the same assay conditions. Insert: qualitative determination of β-galactosidase activity using the chromogenic substrate X-gal (hydrolyzed product was blue). Mean ± DS, N = 3