Table 1 Strains and plasmids used in this study
Strain (plasmid) | Genotype | References |
|---|---|---|
E. coli | ||
JME547 (JMP547) | pUB4-Cre-Hyg | [23] |
JME1046 (JMP1046) | JMP62-URA3ex-pTEF | [34] |
JME3267 (JMP3267) | PUT lys5::URA3ex | This work |
JME3739 (JMP3739) | JMP62-URA3ex-pTEF-CalB | This work |
RIE124 (RIP124) | PUT eyk1::URA3ex | [33] |
JME4001 (JMP4001) | JMP62-URA3ex-pHU8EYK-YFP | Unpublished |
JME4123 (JMP4123) | pUC57-pEYK1-3AB | [19] |
JME4230 (JMP4230) | JMP62-URA3ex-pHU8EYK | This work |
JME4243 (JMP4243) | JMP62-URA3ex-pHU8EYK CalB | This work |
RIE132 (RIP132) | pGEMT-easy-Cre-EYK1 | [33] |
JME4266 (JMP4266) | JMP62-URA3ex-pEYK1-3AB | This work |
JME4365 (JMP4365) | JMP62-URA3ex-pEYK1-3AB-CalB | This work |
JME4579 (JMP4579) | JMP62-LYS5ex-pEYK1-3AB-CalB | This work |
JME4590 (JMP4590) | JMP62-URA3ex-pEYD1-CalB | This work |
RIE279 (RIP279) | JMP62-LYS5ex | This work |
Y. lipolytica | ||
JMY1212 | MATA ura3-302 leu2-270-LEU2-Zeta, xpr2-322, lip2Δ, lip7Δ, lip8Δ | [30] |
JMY5207 | MATA ura3-302 leu2-270-LEU2-Zeta, xpr2-322, lip2Δ, lip7Δ, lip8Δ, lys5::URA3ex | This work |
JMY7121 | MATA ura3-302 leu2-270-LEU2-Zeta, xpr2-322, lip2Δ, lip7Δ, lip8Δ, lys5Δ | This work |
JMY7123 | MATA ura3-302 leu2-270-LEU2-Zeta, xpr2-322, lip2Δ, lip7Δ, lip8Δ, lys5Δ, eyk1::URA3ex | This work |
JMY7126 | MATA ura3-302 leu2-270-LEU2-Zeta, xpr2-322, lip2Δ, lip7Δ, lip8Δ, lys5Δ, eyk1Δ | This work |
JMY7536 | JMY7126 + pTEF-CalB-URA3ex | This work |
JMY7539 | JMY7126 + pEYK1-3AB-CalB-URA3ex | This work |
JMY7544 | JMY7126 + pHU8EYK-CalB-URA3ex | This work |
JMY7548 | JMY7126 + pEYD1-CalB-URA3ex | This work |
RIY368 | JMY7539 + LYS5ex | This work |
RIY394 | JMY7536 + LYS5ex | This work |
RIY406 | JMY7126 + pEYK1-3AB-CalB-URA3ex + pEYK1-3AB-CalB-LYS5ex | This work |