Fig. 1
From: Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries
Schematic representation of bicistronic Fab template constructs. Braces with arrows point the recognition sites of the restriction enzymes BglII, BamHI and NotI present in the templates Fab0 N, Fab0H and Fab0C, respectively. Positions of Lac promoter, translation initiation region of the light chain, start position of the light chain PelB and the start position of the heavy chain PelB are indicated by dashed lines. N n-region, Hydrophobic hydrophobic region and C c-region