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Table 1 Log10 CFU/mL, pH and glucose, fructose concentrations after 3 h of dextransucrase release

From: A systematic approach to study the pH-dependent release, productivity and product specificity of dextransucrases

  Smp./pH Log10 CFU/mL 3 h pH 3 h Glucose 3 h [mM] Fructose 3 h [mM] Glucose 27 h [mM] Fructose 27 h [mM] Protein [µg/mL]
Setup A 3.5 9.69 ± 0.17 3.37 ± 0.01 4.84 ± 0.02 4.90 ± 0.07 12.00 ± 0.29 14.69 ± 0.04 16.38 ± 2.11
4 9.61 ± 0.03 3.88 ± 0.01 4.91 ± 0.08 8.41 ± 0.19 12.07 ± 0.43 30.04 ± 1.00 16.39 ± 1.87
4.5 9.63 ± 0.13 4.38 ± 0.00 5.03 ± 0.05 8.50 ± 0.32 12.18 ± 1.02 29.86 ± 0.07 16.40 ± 1.90
5 9.64 ± 0.09 4.77 ± 0.01 5.04 ± 0.03 7.77 ± 0.36 12.16 ± 1.29 25.74 ± 0.52 16.27 ± 1.98
5.5 9.71 ± 0.10 5.25 ± 0.01 4.72 ± 0.13 7.07 ± 0.30 11.34 ± 0.72 20.88 ± 1.73 15.83 ± 2.02
6 9.62 ± 0.10 5.73 ± 0.01 5.22 ± 0.13 7.27 ± 0.11 11.77 ± 0.62 19.81 ± 0.75 17.12 ± 2.00
6.5 9.67 ± 0.05 6.21 ± 0.00 4.89 ± 0.03 5.83 ± 0.20 11.78 ± 0.02 15.21 ± 0.51 16.60 ± 1.88
Setup B 3.5   4.37 ± 0.01 4.84 ± 0.07 8.40 ± 0.14 11.86 ± 0.32 17.94 ± 0.35  
4   4.37 ± 0.01 4.93 ± 0.02 8.58 ± 0.22 12.04 ± 0.37 30.04 ± 0.51  
4.5   4.37 ± 0.01 5.03 ± 0.10 8.44 ± 0.54 12.35 ± 0.26 30.49 ± 0.85  
5   4.37 ± 0.01 5.16 ± 0.06 8.28 ± 0.46 12.40 ± 0.97 28.66 ± 0.73  
5.5   4.37 ± 0.01 4.99 ± 0.13 8.56 ± 0.57 11.64 ± 0.82 26.99 ± 0.37  
6   4.37 ± 0.01 4.93 ± 0.09 7.90 ± 0.41 12.06 ± 0.69 23.71 ± 1.09  
6.5   4.37 ± 0.01 4.71 ± 0.08 8.27 ± 0.87 11.79 ± 0.20 18.24 ± 0.22  
  1. For calculation of the totally produced dextran amounts, glucose and fructose concentrations were additionally determined at the end of dextran production (27 h). Sugar concentrations were determined according to 2.4. The protein concentrations of the buffer supernatants were determined by the Bradford assay. Data are expressed with mean ± SD of three biological replicates, respectively
  2. Smp. sample