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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Engineering protein production by rationally choosing a carbon and nitrogen source using E. coli BL21 acetate metabolism knockout strains

Fig. 3

Maximal extracellular acetate concentrations. a Maximal acetate concentrations detected for E. coli BL21 wt and knockout strains growing in MM9 or TB7 medium supplemented with glucose or glycerol as the carbon source. b Acetate extracellular concentration percentage relative to E. coli BL21 wt growing on glucose-TB7. c Extracellular acetate concentrations/qs ratio expressed as a percentage relative to E. coli BL21 wt growing on glucose-TB7. Statistical tests, including two-way ANOVA (a and c) and one-way ANOVA (b), were carried out in order to evaluate differential significance between knockout mutants and the wt strain in each condition (a and c), and the differential significance between glucose-TB7 and the other media in the wt strain (b) (p-value < 0.0001 (****), < 0.001 (***), < 0.01 (**), < 0.05(*))

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