Table 1 Biochemical characteristics of free and immobilized organophosphorus hydrolase OPAA4301
Enzyme | Topt (°C) | pHopt | Specific activity (U/mg) | kcat (s−1) | Km (mM) | kcat/Km (M−1/s) | Ratio of target enzyme (%) |
|---|---|---|---|---|---|---|---|
OPAA4301 | 55 | 8.5 | 1.648 ± 0.0222 | 3.0 ± 0.526 | 3.203 ± 0.929 | 935 ± 89 | – |
CI-OPAA | 55 | 8.5 | 0.096 ± 0.0047 | 11.904 ± 3.893 | 6.188 ± 2.490 | 1961 ± 138 | 4.726 |
NCI-OPAA | 50 | 8.0 | 0.109 ± 0.0014 | 11.223 ± 1.752 | 6.116 ± 1.299 | 1850 ± 104 | 5.606 |
CNCI-OPAA | 55 | 8.0 | 0.112 ± 0.0044 | – | – | – | 6.850 |