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Table 1 Biochemical characteristics of free and immobilized organophosphorus hydrolase OPAA4301

From: In vivo immobilization of an organophosphorus hydrolyzing enzyme on bacterial polyhydroxyalkanoate nano-granules

Enzyme Topt (°C) pHopt Specific activity (U/mg) kcat (s−1) Km (mM) kcat/Km (M−1/s) Ratio of target enzyme (%)
OPAA4301 55 8.5 1.648 ± 0.0222 3.0 ± 0.526 3.203 ± 0.929 935 ± 89
CI-OPAA 55 8.5 0.096 ± 0.0047 11.904 ± 3.893 6.188 ± 2.490 1961 ± 138 4.726
NCI-OPAA 50 8.0 0.109 ± 0.0014 11.223 ± 1.752 6.116 ± 1.299 1850 ± 104 5.606
CNCI-OPAA 55 8.0 0.112 ± 0.0044 6.850