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Fig. 3 | Microbial Cell Factories

Fig. 3

From: In vivo immobilization of an organophosphorus hydrolyzing enzyme on bacterial polyhydroxyalkanoate nano-granules

Fig. 3

The SDS-PAGE analysis of PHA granule-associated proteins. Lane M, protein molecular weight standard; Lane 1, purified organophosphorus hydrolase enzyme OPAA4301; Lane 2, PHA granules isolated from recombinant E. coli harboring pACYC-phaAB and pETD-phaC; Lane 3, PHA granules isolated from recombinant E. coli harboring pACYC-phaAB and pETD-opaaC; Lane 4, PHA granules isolated from recombinant E. coli harboring pACYC-phaAB and pETD-CP3opaa, Lane 5, PHA granules isolated from recombinant E. coli harboring pACYC-phaAB and pETD-P3opaa. (FP, phaC, and OPAA indicate the corresponding bands of fusion proteins, PHA synthetase, and the enzyme OPAA4301, respectively)

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