Metabolic fluxes-oriented control of bioreactors: a novel approach to tune micro-aeration and substrate feeding in fermentations

Table 1 Performance indexes for controlled fermentation FMC and for the reference fermentation strategies (BBP and SAC)

Performance indexes	FMC	BBP	SAC
Ethanol (g L⁻¹)	87 ± 2	62.1 ± 0.3	57.2 ± 0.8
Glycerol (g L⁻¹)	8.76 ± 0.01	9.33 ± 0.02	9.3 ± 0.1
Y_x/s (g_cells g ⁻¹_substrate )	0.11 ± 0.001	0.08 ± 0.01	0.08 ± 0.01
Y_EtOH/s (g_ethanol g ⁻¹_substrate )	0.46 ± 0.01	0.38 ± 0.03	0.39 ± 0.02
Pr_x (g L⁻¹ h⁻¹)	1.2 ± 0.2	0.59 ± 0.01	0.77 ± 0.01
Pr_p,Ethanol (g L⁻¹ h⁻¹)	7.0 ± 0.2	3.75 ± 0.02	4.05 ± 0.02
S_Index	9.9 ± 0.8	6.6 ± 0.1	6.2 ± 0.7
Total glucose mass fed (g)	616 ± 4	552 ± 2	544 ± 2
Conversion (%)	99.6 ± 0.4	97.88 ± 0.02	99.00 ± 0.02

Ethanol and glycerol concentrations at the end of the cultivation. Overall cultivation parameters estimated: Y_x/s biomass yield, Y_EtOH/S ethanol yield, Pr_X volumetric cell productivity, Pr_p,Ethanol volumetric ethanol productivity, S_Index selectivity index, S_Total total glucose mass fed

ISSN: 1475-2859