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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds

Fig. 1

Schematic diagram of genotypes of R. eutropha NSDG-GG-based recombinant strains. phaCNSDG, a gene encoding N149S/D171G mutant of PHA synthase from A. caviae; phaA, β-ketothiolase gene; phaB1, a gene encoding major NADPH-acetoacetyl-CoA reductase; phaRRe, PHA-binding transcriptional repressor gene; phaB1NV and phaB1NVYF, genes encoding N142V and N142V/Y185F mutants of PhaB1; phaB2, a gene encoding minor NADPH-acetoacetyl-CoA reductase; had, NAD-(S)-3HB-CoA dehydrogenase gene, crt2, crotonase gene. The commonly modified loci in these strains are shown in Additional file 1: Fig. S1

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