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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolic engineering of Bacillus megaterium for heparosan biosynthesis using Pasteurella multocida heparosan synthase, PmHS2

Fig. 2

Screening of B. megaterium colonies. a Various colonies from successful B. megaterium transformations screened for heparosan production, where 0S-HS refers to the unsulfated heparosan disaccharide units as quantified by LCMS analysis. A1, A2: colonies of negative control strain harboring pPT7_X plasmid; B1, B2, B3, B4: colonies harboring the pPT7_PmHS2 plasmid. b Heparosan titers from shake flask control experiment of the pPT7_PmHS2 strain (B3) induced and uninduced, and pPT7_X strain (A1) induced

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