Fig. 3

Resting cell bioconversion analysis of recombinant C. crenatum in 5 L bioreactor. Recombinant C. crenatum (200 µL) was transferred to 20 mL LBG medium and incubated for 24 h at 180 r min⁻¹, 30 °C. After the 10% cultures were transferred and cultured in 200 mL seed medium for 18 h, the whole cultures were transferred to 5 L bioreactor containing 2 L of fermentation medium, and incubated for 24 h at 30 °C, 600 r min⁻¹. 4 L recombinant C. crenatum cultures were collected and resuspended in 2 L resting cell bioconversion medium containing 100 g L⁻¹ glucose. Resting cell bioconversion was performed in batches at 30 °C, 250 r min⁻¹. Consumed glucose, formed metabolites, and organic acid during the bioconversion of resting cells are shown. The results of resting cell bioconversion kinetics are shown as the mean ± standard of three replicates