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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Fig. 3

Resting cell bioconversion analysis of recombinant C. crenatum in 5 L bioreactor. Recombinant C. crenatum (200 µL) was transferred to 20 mL LBG medium and incubated for 24 h at 180 r min−1, 30 °C. After the 10% cultures were transferred and cultured in 200 mL seed medium for 18 h, the whole cultures were transferred to 5 L bioreactor containing 2 L of fermentation medium, and incubated for 24 h at 30 °C, 600 r min−1. 4 L recombinant C. crenatum cultures were collected and resuspended in 2 L resting cell bioconversion medium containing 100 g L−1 glucose. Resting cell bioconversion was performed in batches at 30 °C, 250 r min−1. Consumed glucose, formed metabolites, and organic acid during the bioconversion of resting cells are shown. The results of resting cell bioconversion kinetics are shown as the mean ± standard of three replicates

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