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Table 2 Oligonucleotides used in this study

From: Metabolic engineering of roseoflavin-overproducing microorganisms

Oligo name Sequence (5′ to 3′)a Restriction sites Function
RoF_BamHI—Fw tct aga gga tcc gat ggc tct taa agc tct tat cct taa c BamHI Vector pRML2 construction
RoF_SalI—Rv tat ggg tcg act taa tgg ccg ttc atg att tta gat tta g SalI
RoF_NcoI—Fw cag agc cca tgg ctc tta aag ctc tta tcc tta aca caa c NcoI Vector pRML3 construction
RoF_KpnI—Rv tcg agg gta cct taa tgg ccg ttc atg att tta gat tta gaa ac KpnI
ribM_NdeI_SpeI—Fw ttt aac ttt aag aag gag ata tac ata tga cta gta tga att ggc tga ata gc NdeI Vector pRML4 construction
ribM_PacI_NcoI—Rv agg ata aga gct tta aga gcc atg gat cct tcc tcc ttt tta att aat tag tgg tgg tga tgg tg NcoI
pHT01 seq fw agc gga aaa gaa tga tgt aag cgt PCR and sequence analysis of pRML1
pHT01 seq rev tcc taa taa gcc gat att agc ctc
lacA 5′—Fw tat cag ggc ctc gac tac agc PCR and sequence analysis of pRML2
lacA 3′—Rv cgt ttc aag agg ctc aac tcc
pMKEx2 01—Fw act cct gca tta gga agc agc PCR and sequence analysis of pRML3 and pRML4
pMKEx2 01—Rv ttt tag cta tct gtc gca gcg
Pseq1_fw cga tta agt tgg gta acg cca gg PCR and sequence analysis of pSETPermErosArosB
Pseq7_rev gca gtg agc gca acg caa tta atg
rosB_dDIGa ctt tgc ctt cga tgt agc cag ggc ctg tgc Northern blot analysis of C. glutamicum transformants
  1. aRestriction endonuclease sequences are highlighted in bold. The rosB_dDIG probe was ordered with a dual 5′/3′ digoxigenin (DIG) label from Biomers.net GmbH (Ulm/Donau, Germany)