Table 2 Oligonucleotides used in this study

RoF_BamHI—Fw

tct aga gga tcc gat ggc tct taa agc tct tat cct taa c

BamHI

Vector pRML2 construction

RoF_SalI—Rv

tat ggg tcg act taa tgg ccg ttc atg att tta gat tta g

SalI

RoF_NcoI—Fw

cag agc cca tgg ctc tta aag ctc tta tcc tta aca caa c

NcoI

Vector pRML3 construction

RoF_KpnI—Rv

tcg agg gta cct taa tgg ccg ttc atg att tta gat tta gaa ac

KpnI

ribM_NdeI_SpeI—Fw

ttt aac ttt aag aag gag ata tac ata tga cta gta tga att ggc tga ata gc

NdeI

Vector pRML4 construction

ribM_PacI_NcoI—Rv

agg ata aga gct tta aga gcc atg gat cct tcc tcc ttt tta att aat tag tgg tgg tga tgg tg

NcoI

pHT01 seq fw

agc gga aaa gaa tga tgt aag cgt

–

PCR and sequence analysis of pRML1

pHT01 seq rev

tcc taa taa gcc gat att agc ctc

–

lacA 5′—Fw

tat cag ggc ctc gac tac agc

–

PCR and sequence analysis of pRML2

lacA 3′—Rv

cgt ttc aag agg ctc aac tcc

–

pMKEx2 01—Fw

act cct gca tta gga agc agc

–

PCR and sequence analysis of pRML3 and pRML4

pMKEx2 01—Rv

ttt tag cta tct gtc gca gcg

–

Pseq1_fw

cga tta agt tgg gta acg cca gg

–

PCR and sequence analysis of pSETPermErosArosB

Pseq7_rev

gca gtg agc gca acg caa tta atg

–

rosB_dDIG^a

ctt tgc ctt cga tgt agc cag ggc ctg tgc

–

Northern blot analysis of C. glutamicum transformants