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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Metabolic engineering of roseoflavin-overproducing microorganisms

Fig. 4

The enzymes RFK, RosB and RosA are active in recombinant Corynebacterium glutamicum strains as shown by HPLC analysis of the resulting specific reaction products (structures shown above the graphs). Cell-free extracts of IPTG-induced Corynebacterium glutamicum strains (harvested 6 h after induction) were used in different enzyme assays. HPLC-based analysis revealed signals of the assay substrates (marked with a plus +) and the expected reaction products (marked with a star *) for RFK (left graph), RosB (center graph) and RosA (right graph). a Control strain C5–6 (negative control), b recombinant strain RML5, c recombinant strain RML6 and d purified recombinant protein (positive control)

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