Fig. 4
From: Metabolic engineering of roseoflavin-overproducing microorganisms
The enzymes RFK, RosB and RosA are active in recombinant Corynebacterium glutamicum strains as shown by HPLC analysis of the resulting specific reaction products (structures shown above the graphs). Cell-free extracts of IPTG-induced Corynebacterium glutamicum strains (harvested 6 h after induction) were used in different enzyme assays. HPLC-based analysis revealed signals of the assay substrates (marked with a plus +) and the expected reaction products (marked with a star *) for RFK (left graph), RosB (center graph) and RosA (right graph). a Control strain C5–6 (negative control), b recombinant strain RML5, c recombinant strain RML6 and d purified recombinant protein (positive control)