Fig. 4

Differential expression of selected genes in fructose- (dark grey) or glucose- (light grey) grown cultures. Relative gene expression was analyzed by RT-PCR of RNA samples were taken from cultures at the exponential phase of growth. ΔΔC_T values were calculated taking glycerol-grown cultures as reference condition and the results are expressed as \(2^{{ - \Delta \Delta {\text{C}}_{\text{T}} }}\), calculated as the averages of at least two biological replicates and three technical replicates. The polA and dnaA genes from C. salexigens, encoding DNA polymerase I and DNA-binding transcriptional dual regulator, respectively, were used as internal control for relative quantification. See “Methods” for further details. The abbreviations for the names of the enzymes are given in Fig. 3 legend. Csal2648 sequence comprises domains orthologue to subunits EI, HPr, and EIIA of the fructose specific phosphotransferase system (Pts^Fru). Csal2646 sequence comprises domains orthologue to subunits EIIB and, EIIC of the fructose specific phosphotransferase system (Pts^Fru). *Automatic assignments still needing experimental evidence