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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

Fig. 4

Differential expression of selected genes in fructose- (dark grey) or glucose- (light grey) grown cultures. Relative gene expression was analyzed by RT-PCR of RNA samples were taken from cultures at the exponential phase of growth. ΔΔCT values were calculated taking glycerol-grown cultures as reference condition and the results are expressed as \(2^{{ - \Delta \Delta {\text{C}}_{\text{T}} }}\), calculated as the averages of at least two biological replicates and three technical replicates. The polA and dnaA genes from C. salexigens, encoding DNA polymerase I and DNA-binding transcriptional dual regulator, respectively, were used as internal control for relative quantification. See “Methods” for further details. The abbreviations for the names of the enzymes are given in Fig. 3 legend. Csal2648 sequence comprises domains orthologue to subunits EI, HPr, and EIIA of the fructose specific phosphotransferase system (PtsFru). Csal2646 sequence comprises domains orthologue to subunits EIIB and, EIIC of the fructose specific phosphotransferase system (PtsFru). *Automatic assignments still needing experimental evidence

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