Table 3 Comparison of integration efficiency via homologous recombination by pR6K-Cm-11after screening at different Cm concentration in DK-2566
Strain | Cm (μg/ml) | Number of Cm resistance (three replication) | Number of PCR positive by primer P1 and P2 per 100 colony | PCR positive by primer P3 and P4 (%) | PCR positive by primer P5 and P6 (%) | ||
|---|---|---|---|---|---|---|---|
DK1622 | 10 | 100 | 0 | 0 | 0 | 100 | 0 |
DK-2566 | 8 | 100 | 19 | 21 | 22 | 100 | 0 |
12 | 100 | 19 | 20 | 23 | 100 | 0 | |
16 | 100 | 18 | 16 | 25 | 100 | 0 | |