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Table 3  Comparison of integration efficiency via homologous recombination by pR6K-Cm-11after screening at different Cm concentration in DK-2566

From: Whole transcriptome analysis and gene deletion to understand the chloramphenicol resistance mechanism and develop a screening method for homologous recombination in Myxococcus xanthus

Strain Cm (μg/ml) Number of Cm resistance (three replication) Number of PCR positive by primer P1 and P2 per 100 colony PCR positive by primer P3 and P4 (%) PCR positive by primer P5 and P6 (%)
DK1622 10 100 0 0 0 100 0
DK-2566 8 100 19 21 22 100 0
12 100 19 20 23 100 0
16 100 18 16 25 100 0