Fig. 4From: Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymesAnalysis of UDP-Glc formation. a The supernatant of reaction catalyzed by the hybrid cascade system comprised of separate whole cell catalysts expressing TmαGP and StUSP, in the optimized conditions described above, was assayed by PAMN-HPLC. b ESI–MS spectrum of product UDP-Glc. The supernatant of reaction catalyzed by the hybrid cascade system comprised of separate whole cell catalysts expressing TmαGP and StUSP, in the optimized conditions described above, was assayed by MS after desalting using a P2 columnBack to article page