Fig. 4From: A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloidesExpression of EGFP and mRuby2 from bidirectional promoters. Fluorescence of EGFP and mRuby2 for each bidirectional promoter pair was measured in strains harboring promoter constructs in orientation 1. The heatmap was generated using MultiExperiment Viewer (MeV), and promoters were clustered by hierarchical clustering using Euclidean distance. Fluorescence expression values are on a Log2 scale (color scale is shown in the upper bar), calculated as described in “Methods”. Each column represents a time point, shown in hours. Each row represents a promoter, named on the right side of the heatmap. All time points for all media are shown for both EGFP and mRubyBack to article page