Skip to main content

Advertisement

Table 2 Physiological data for the evolved isolates on sucrose M9 medium and sugarcane juice M9 minimal medium (SCJ medium)

From: Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution

Clone/construct Clone ID M9 Sucrose medium SCJ medium
Growth rate, μ (h−1) on M9 2% sucrose Growth rate, μ (h−1) Final density (gCDW/L) Sucrose uptake rate (mmol gCDW−1 h−1) Acetate production rate (mmol gDW−1 h−1) Biomass yield, YX/S, (gCDW g of sucrose−1) Uptake rate fold increase vs start strain
K-12 MGcscBKA Starting strain 0.54 ± 0.01 0.68 ± 0.03 1.47 ± 0.04 7.56 ± 0.96 4.20 ± 0.20 0.28 ± 0.01 1.00
1 0.85 ± 0.01 0.88 ± 0.02 1.91 ± 0.03 8.71 ± 0.2 4.73 ± 0.11 0.30 ± 0.01 1.15
2 0.63 ± 0.01 0.68 ± 0.02 1.52 ± 0.04 6.54 ± 0.23 5.67 ± 0.54 0.30 ± 0.01 0.86
3 0.83 ± 0.01 0.86 ± 0.02 1.68 ± .0.03 7.84 ± 0.18 5.42 ± 0.13 0.32 ± 0.01 1.04
K-12 MGcscBKAp Starting strain 0.64 ± 0.01 0.73 ± 0.01 1.48 ± 0.02 7.01 ± 1.9 4.99 ± 0.25 0.30 ± 0.01  
1 0.86 ± 0.01 0.91 ± 0.01 1.38 ± 0.02 9.89 ± 0.19 6.83 ± 0.01 0.27 ± 0.01 1.41
2 0.88 ± 0.01 0.88 ± 0.01 1.44 ± 0.02 9.50 ± 0.01 8.27 ± 0.01 0.27 ± 0.01 1.36
3 0.80 ± 0.01 0.82 ± 0.01 1.51 ± 0.01 8.98 ± 0.11 7.30 ± 0.09 0.27 ± 0.01 1.28
E. coli W Starting strain 0.90 ± 0.02 0.97 ± 001 1.81 ± 0.05 9.88 ± 0.35 6.60 ± 0.49 0.29 ± .01  
1 1.10 ± 0.01 1.06 ± 0.05 1.68 ± 0.06 10.5 ± 0.47 15.90 ± 0.68 0.29 ± 0.012 1.06
2 0.95 ± 0.01 0.96 ± 0.02 1.74 ± 0.05 14.10 ± 1.0 6.50 ± 0.51 0.20 ± 0.02 1.43
3 0.95 ± 0.01 1.08 ± 0.02 1.92 ± 0.01 12.51 ± 1.1 7.40 ± 0.5 0.25 ± 0.03 1.27
  1. The physiological properties of each of the clones isolated from the independent endpoint ALE experiments were compared to examine whether there were any improved phenotypic outcomes across the different experiments