Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution

Table 2 Physiological data for the evolved isolates on sucrose M9 medium and sugarcane juice M9 minimal medium (SCJ medium)

Clone/construct	Clone ID	M9 Sucrose medium	SCJ medium
Clone/construct	Clone ID	Growth rate, μ (h⁻¹) on M9 2% sucrose	Growth rate, μ (h⁻¹)	Final density (gCDW/L)	Sucrose uptake rate (mmol gCDW⁻¹ h⁻¹)	Acetate production rate (mmol gDW⁻¹ h⁻¹)	Biomass yield, Y_X/S, (gCDW g of sucrose⁻¹)	Uptake rate fold increase vs start strain
K-12 MGcscBKA	Starting strain	0.54 ± 0.01	0.68 ± 0.03	1.47 ± 0.04	7.56 ± 0.96	4.20 ± 0.20	0.28 ± 0.01	1.00
	1	0.85 ± 0.01	0.88 ± 0.02	1.91 ± 0.03	8.71 ± 0.2	4.73 ± 0.11	0.30 ± 0.01	1.15
	2	0.63 ± 0.01	0.68 ± 0.02	1.52 ± 0.04	6.54 ± 0.23	5.67 ± 0.54	0.30 ± 0.01	0.86
	3	0.83 ± 0.01	0.86 ± 0.02	1.68 ± .0.03	7.84 ± 0.18	5.42 ± 0.13	0.32 ± 0.01	1.04
K-12 MGcscBKAp	Starting strain	0.64 ± 0.01	0.73 ± 0.01	1.48 ± 0.02	7.01 ± 1.9	4.99 ± 0.25	0.30 ± 0.01
	1	0.86 ± 0.01	0.91 ± 0.01	1.38 ± 0.02	9.89 ± 0.19	6.83 ± 0.01	0.27 ± 0.01	1.41
	2	0.88 ± 0.01	0.88 ± 0.01	1.44 ± 0.02	9.50 ± 0.01	8.27 ± 0.01	0.27 ± 0.01	1.36
	3	0.80 ± 0.01	0.82 ± 0.01	1.51 ± 0.01	8.98 ± 0.11	7.30 ± 0.09	0.27 ± 0.01	1.28
E. coli W	Starting strain	0.90 ± 0.02	0.97 ± 001	1.81 ± 0.05	9.88 ± 0.35	6.60 ± 0.49	0.29 ± .01
	1	1.10 ± 0.01	1.06 ± 0.05	1.68 ± 0.06	10.5 ± 0.47	15.90 ± 0.68	0.29 ± 0.012	1.06
	2	0.95 ± 0.01	0.96 ± 0.02	1.74 ± 0.05	14.10 ± 1.0	6.50 ± 0.51	0.20 ± 0.02	1.43
	3	0.95 ± 0.01	1.08 ± 0.02	1.92 ± 0.01	12.51 ± 1.1	7.40 ± 0.5	0.25 ± 0.03	1.27

The physiological properties of each of the clones isolated from the independent endpoint ALE experiments were compared to examine whether there were any improved phenotypic outcomes across the different experiments

ISSN: 1475-2859