Fig. 6
Relative transcript amounts of acbC gene under control of different homologous and heterologous promoters in Actinoplanes sp. SE50/110. The RNA was isolated from the growth phase of a shake flask cultivation in maltose minimal medium and analyzed by RT-qPCR. The transcript amounts were analyzed in relation to the empty vector control. Shown are the means and standard deviation of at least three biological replicates. The RT-qPCR indicates significant increase of gene expression compared to the empty vector control (set to a value of 1), which was tested by a two-sided t-test (p-values: PacbV: 0.9503, PrpsJ: 0.6084, Pefp: 7.769e−05, Pcgt: 0.004097, PtipA: 2.958e−06, PrpsL: 1.89e−06, PgapDH: 2.426e−08)