Fig. 6

Lysine productions and PI uptake analyses of LYS1 derived strains. a Lysine productions. b PI uptakes. Control represents LYS1 (pSB). speB represents LYS1 (pSB-speB). speB^C905T represents LYS1 (pSB-speB^C905T). atpB represents LYS1 (pSB-atpB). atpB^G494A represents LYS1 (pSB-atpB^G494A). secY-D represents LYS1 (pSB-sRNA^secY-MicC), which can transcribe a small regulatory RNA (sRNA) to inhibit SecY synthesis. Cells were cultivated for 40 h in 96-deep-well plate with fermentation medium. Data are the mean and standard deviation of independent triplicates. The p value was calculated by Student’s two-tailed t-test. * represents a p value of < 0.05, and ** represents a p value of < 0.01, comparing with the data of control strain