Fig. 5

Intracellular metabolic profiling of intermediates involved in the selected metabolic pathways in RS3. Metabolic modules are marked with color. The modules include The Embden–Meyerhof–Parnas Pathway (EMP), the pentose phosphate pathway (PPP), the tricarboxylic acid cycle (TCA left half and TCA right half), the aspartate family amino acids synthetic pathways (Aspartate family), the redox equivalents and GTP/ATP synthetic pathways (Redox and energy), and the polyamines synthetic pathway (Polyamines). Samples of 9 h fed-batch fermentation were applied for the metabolomics analysis. The arrows indicate the increase (arrow pointing upwards) or decrease (arrow pointing downwards) of metabolites in RS3 compared with MU-11, while the bars without arrow mean almost no change. The values were determined by dividing the LC–MS/MS peak areas of metabolites in RS3 by the corresponding peak areas of metabolites in MU-11. Data are the mean of independent triplicates