Fig. 2

Lysine productions of ALE selected mutants in 96-deep-well plates and flasks. a Lysine production in 96-deep-well plates. Lysine was tested at 16 h. Three colonies from each of the 10 samples were randomly selected and tested. The three colonies in each sample were arranged in the figure according to the lysine production from high to low, their best ones were named as RS1 to RS10. Data for MU-11 are the mean and standard deviation of independent triplicates. b Lysine production of 9 mutants in 500-mL flasks containing 20 mL media. Dark grey bars and light grey bars represent lysine concentration and yield of lysine from glucose, respectively. pH was adjusted at 16 h using 400 μL 25% NH₃·H₂O, and samples were tested at 24 h. Data are the mean and standard deviation of independent triplicates