Fig. 2From: Production of trehalose with trehalose synthase expressed and displayed on the surface of Bacillus subtilis sporesWestern blot analysis of TreS displayed on the spore surface. Spores were incubated with the mouse anti-His tag antibody (dilution 1:3000), followed by probing with a horseradish peroxidase-conjugated goat anti-mouse IgG antibody (1:2000). Lane M: markers; lane 1: CotG-TreS; lane 2: CotC-TreS; lane 3: B. subtilis WB800n control; lane 4: CotC-TreS–CotG-TreSBack to article page