Table 1 Plasmids and strains
Plasmids and strains | Relevant characteristics | References or source |
|---|---|---|
Plasmids | ||
 pAX01 | B. subtilis integration vector, xylr-Pxyl cassette, Ampr, Ermr | Lab stock |
 pAX-comK | Derived from pAX01, comK inserted | This study |
 pBP43GFP | E. coli-B. subtilis shuttle vector, P43 promoter, gfp, Ampr, Kanr | Lab stock |
 pBSG03 | E. coli-B. subtilis shuttle vector, PsrfA promoter, gfp, Ampr, Kanr | Lab stock |
 pBS-gusA | gusA expressed by wild type PsrfA | This study |
 pBBH4-gusA | gusA expressed by mutant promoter PBH4 | This study |
 pBS-NK | Secretion expression of nattokinase by wild type PsrfA and WapA signal peptide | This study |
 pBBH4-NK | Secretion expression of nattokinase by wild type PBH4 and WapA signal peptide | This study |
 pBPylbP-GFP | Derived from pBSG03, PsrfA::PylbP-RBS, gfp, Ampr, Kanr | This study |
 pBPxylA-GFP | Derived from pBSG03, PsrfA::PxylA, gfp, Ampr, Kanr | This study |
Strains | ||
 E.coli JM109 | recA1, supE44 endA1 hsdR17 (r−k,m+ k) gyrA96 relA1 thi (lac-proAB) F’[traD36 proAB+ lacIq lacZ ΔM15] | Lab stock |
 B. subtilis 168 | trpC2 | Lab stock |
 B. subtilis comK | Derived from B. subtilis 168, lacA::PxylA-comK, Ermr | This study |
 B. subtilis 3NA | spo0A3 | Lab stock |
 BSGus | B. subtilis comK harboring pBS-gusA | This study |
 BSBHGus | B. subtilis comK harboring pBBH4-gusA | This study |
 BSNK | B. subtilis 3NA harboring pBS-NK | This study |
 BSBHNK | B. subtilis 3NA harboring pBBH4-NK | This study |