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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis

Fig. 4

Heterologous protein expression in the shake flask condition identified the compatibility of the evolved promoter. a The growth curves and GusA expression levels of BSGus and BSBHGus at different culture times were measured and determined to show the promoter strength and the compatibility to the Gus protein. The data are shown as the mean ± S.D. All the experiments were performed in triplicate. b SDS-PAGE analysis confirmed the expression level of GusA. The treatment of the samples was performed as aforementioned. CK denoted the strain without any plasmid. c The growth curves and expression levels of NK of BSNK and BSBHNK, at the different culture times, were measured to identify the compatibility and stability of BH4 with the host. The data are shown as the mean ± S.D. All the experiments were performed in triplicate. d The expression levels of NK were determined by SDS-PAGE analysis after 6-, 12-, and 24-h culture. The treatment of each sample was performed as aforementioned. CK denoted the strain without any plasmid

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Microbial Cell Factories

ISSN: 1475-2859

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