Table 1 Strains, plasmids and primers used in this study
From: Co-production of farnesol and coenzyme Q10 from metabolically engineered Rhodobacter sphaeroides
| Â | Description | References |
|---|---|---|
Strain | ||
 E. coli S17-1 | RP4-2. Tc::Mu-Km::Tn7 | [27] |
 E. coli DH5α | F-λ-endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK-mK+) | Invitrogen |
 E. coli TOP10 | F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 | Invitrogen |
|  | Δ(ara-leu)7697 galU galK λ-rpsL(StrR) endA1 nupG | Invitrogen |
 R. sphaeroides GY-2 | CoQ10 high-yield mutant strain obtained from R. sphaeroides 2.4.1 | This study |
 Rs-TPS | R. sphaeroides GY-2 harboring TPS | This study |
 Rs-ATPS | R. sphaeroides GY-2 harboring ATPS | This study |
 Rs-PgpB | R. sphaeroides GY-2 harboring PgpB | This study |
 PSYi1, PSYi2, PSYi3, PSYi4 | R. sphaeroides GY-2 derivatives with psy RNA interference | This study |
 TPS-PSYi | R. sphaeroides GY-2 harboring TPS with psy RNA interference | This study |
 ATPS-PSYi | R. sphaeroides GY-2 harboring ATPS with psy RNA interference | This study |
 PgpB-PSYi | R. sphaeroides GY-2 harboring PgpB with psy RNA interference | This study |
Plasmids | ||
 pYC6a | Ptac promoter, AmpR | This study |
 pBBR1MCS2 | low-copy cloning vector, KanR | [28] |
 pBBR1MCS2-tac | pBBR1MCS2 containing tac promoter | This study |
 pBBR1MCS2-PgpB | pBBR1MCS2-tac containing PgpB from E. coli | This study |
 pBBR1MCS2-ATPS | pBBR1MCS2-tac containing ATPS from Brachypodium distachyon | This study |
 pBBR1MCS2-TPS | pBBR1MCS2-tac containing TPS from Zea mays | This study |
 pBBR1MCS2-PSYi | pBBR1MCS2-tac containing PSYi | This study |
 pBBR1MCS2-PSYi-PgpB | pBBR1MCS2-PSYi containing PgpB from E. coli | This study |
 pBBR1MCS2-PSYi-ATPS | pBBR1MCS2-PSYi containing ATPS from Brachypodium distachyon | This study |
 pBBR1MCS2-PSYi-TPS | pBBR1MCS2-PSYi containing TPS from Zea mays | This study |
Primers | Sequence (5′–3′) |  |
|---|---|---|
PTR-F | ATTCCCCGCGGCACAGCTAACACCACGTCGTC | Â |
PTR-R | GAGCCCAAGCTTGAAAGGCCCAGTCTTTCGAC | Â |
PSYLi-F | GGAATTCCGGCGGCGATGGCCGCGCCCC | Â |
PSYLi-R | TTCCGCGGCCGCTATGGCCGACGTCGACGCCAGGCCGCGAGCCCCGCGG | Â |
PSYSi-F | GGAATTCCCTCGAGGTGGCGCGCACGCC | Â |
PSYSi-R | CGAGCTCGCCAGGCCGCGAGCCCCGC | Â |
pBBR1MCS2-F | GAATTCGGTGAGCTCGGTCTG | Â |
pBBR1MCS2-R | GGTTAATTCCTCCTGTTACGCGC | Â |
PgpB-EcoF | CGTAACAGGAGGAATTAACCATGCGTTCGATTGCCAGAC | Â |
PgpB-EcoR | AGACCGAGCTCACCGAATTCTTAGTGGTGGTGGTGGTGGTG | Â |
ATPS-F | CGTAACAGGAGGAATTAACCATGCACATCGACCCGGCC | Â |
ATPS-R | AGACCGAGCTCACCGAATTCTTAGAGCGAGTCCGAGAAGTCG | Â |
TPS-F | CGTAACAGGAGGAATTAACCATGGCCATGCCGGTGAAG | Â |
TPS-R | AGACCGAGCTCACCGAATTCTTACACGTCCGACACGAG | Â |
PSYitac-F | CGCGGATCCCACAGCTAACACCACGTCG | Â |
PSYiT-R | TTGGCGCGCCTAGTGGTGGTGGTGGTGGT | Â |