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Table 1 Strains, plasmids and primers used in this study

From: Co-production of farnesol and coenzyme Q10 from metabolically engineered Rhodobacter sphaeroides

 

Description

References

Strain

 E. coli S17-1

RP4-2. Tc::Mu-Km::Tn7

[27]

 E. coli DH5α

F-λ-endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15

Δ(lacZYA-argF)U169, hsdR17(rK-mK+)

Invitrogen

 E. coli TOP10

F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139

Invitrogen

 

Δ(ara-leu)7697 galU galK λ-rpsL(StrR) endA1 nupG

Invitrogen

 R. sphaeroides GY-2

CoQ10 high-yield mutant strain obtained from R. sphaeroides 2.4.1

This study

 Rs-TPS

R. sphaeroides GY-2 harboring TPS

This study

 Rs-ATPS

R. sphaeroides GY-2 harboring ATPS

This study

 Rs-PgpB

R. sphaeroides GY-2 harboring PgpB

This study

 PSYi1, PSYi2, PSYi3, PSYi4

R. sphaeroides GY-2 derivatives with psy RNA interference

This study

 TPS-PSYi

R. sphaeroides GY-2 harboring TPS with psy RNA interference

This study

 ATPS-PSYi

R. sphaeroides GY-2 harboring ATPS with psy RNA interference

This study

 PgpB-PSYi

R. sphaeroides GY-2 harboring PgpB with psy RNA interference

This study

Plasmids

 pYC6a

Ptac promoter, AmpR

This study

 pBBR1MCS2

low-copy cloning vector, KanR

[28]

 pBBR1MCS2-tac

pBBR1MCS2 containing tac promoter

This study

 pBBR1MCS2-PgpB

pBBR1MCS2-tac containing PgpB from E. coli

This study

 pBBR1MCS2-ATPS

pBBR1MCS2-tac containing ATPS from Brachypodium distachyon

This study

 pBBR1MCS2-TPS

pBBR1MCS2-tac containing TPS from Zea mays

This study

 pBBR1MCS2-PSYi

pBBR1MCS2-tac containing PSYi

This study

 pBBR1MCS2-PSYi-PgpB

pBBR1MCS2-PSYi containing PgpB from E. coli

This study

 pBBR1MCS2-PSYi-ATPS

pBBR1MCS2-PSYi containing ATPS from Brachypodium distachyon

This study

 pBBR1MCS2-PSYi-TPS

pBBR1MCS2-PSYi containing TPS from Zea mays

This study

Primers

Sequence (5′–3′)

 

PTR-F

ATTCCCCGCGGCACAGCTAACACCACGTCGTC

 

PTR-R

GAGCCCAAGCTTGAAAGGCCCAGTCTTTCGAC

 

PSYLi-F

GGAATTCCGGCGGCGATGGCCGCGCCCC

 

PSYLi-R

TTCCGCGGCCGCTATGGCCGACGTCGACGCCAGGCCGCGAGCCCCGCGG

 

PSYSi-F

GGAATTCCCTCGAGGTGGCGCGCACGCC

 

PSYSi-R

CGAGCTCGCCAGGCCGCGAGCCCCGC

 

pBBR1MCS2-F

GAATTCGGTGAGCTCGGTCTG

 

pBBR1MCS2-R

GGTTAATTCCTCCTGTTACGCGC

 

PgpB-EcoF

CGTAACAGGAGGAATTAACCATGCGTTCGATTGCCAGAC

 

PgpB-EcoR

AGACCGAGCTCACCGAATTCTTAGTGGTGGTGGTGGTGGTG

 

ATPS-F

CGTAACAGGAGGAATTAACCATGCACATCGACCCGGCC

 

ATPS-R

AGACCGAGCTCACCGAATTCTTAGAGCGAGTCCGAGAAGTCG

 

TPS-F

CGTAACAGGAGGAATTAACCATGGCCATGCCGGTGAAG

 

TPS-R

AGACCGAGCTCACCGAATTCTTACACGTCCGACACGAG

 

PSYitac-F

CGCGGATCCCACAGCTAACACCACGTCG

 

PSYiT-R

TTGGCGCGCCTAGTGGTGGTGGTGGTGGT

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