Table 1 The amino acid sequence of SAPs designed in this study
From: An efficient expression tag library based on self-assembling amphipathic peptides
Schemes | SAPs | Amino acid sequence | GRAVYa | Net charge |
|---|---|---|---|---|
S1 | (AEAEAKAK)2 | − 0.95 | 0 | |
Hydrophobic residues | S1hv1 | (IEIEIKIK)2 | 0.4 | 0 |
S1hv2 | (LELELKLK)2 | 0.05 | 0 | |
S1hv3 | (VEVEVKVK)2 | 0.25 | 0 | |
S1hv4 | (FEFEFKFK)2 | − 0.45 | 0 | |
S1hv5 | (GEGEGKGK)2 | − 2.05 | 0 | |
S1hv6 | (PEPEPKPK)2 | − 2.65 | 0 | |
Hydrophilic residues | S1cv1 | (ADADAHAH)2 | − 0.78 | 0 |
S1cv2 | (AEAEAHAH)2 | − 0.78 | 0 | |
S1cv3 | (ADADAKAK)2 | − 0.95 | 0 | |
S1cv4 | (AEAEARAR)2 | − 1.1 | 0 | |
S1cv5 | (ADADARAR)2 | − 1.1 | 0 | |
Length | S1lv1 | AEAEAKAK | − 0.95 | 0 |
S1lv2 | (AEAEAKAK)3 | − 0.95 | 0 | |
S1lv3 | (AEAEAKAK)4 | − 0.95 | 0 | |
S1lv4 | (AEAEAKAK)5 | − 0.95 | 0 | |
S1lv5 | (AEAEAKAK)6 | − 0.95 | 0 | |
S1lv6 | (AEAEAKAK)7 | − 0.95 | 0 | |
S1lv7 | (AEAEAKAK)8 | − 0.95 | 0 | |
S1lv8 | (AEAEAKAK)9 | − 0.95 | 0 | |
S1lv9 | (AEAEAKAK)10 | − 0.95 | 0 | |
Net charge | S1nv1 | (ANANARAR)10 | − 1.1 | + 20 |
S1nv2 | (ANANADAD)10 | − 1.1 | − 20 | |
S1nv3 | (ANANADAD)8 | − 1.1 | − 16 | |
S1nv4 | (ANANADAD)7 | − 1.1 | − 14 | |
S1nv5 | (ANANADAD)6 | − 1.1 | − 12 | |
S1nv6 | (ANANADAD)4 | − 1.1 | − 8 | |
S1nv7 | (ANANADAD)3 | − 1.1 | − 6 | |
S1nv8 | (ANANADAD)2 | − 1.1 | − 4 | |
S1nv9 | ANANADAD | − 1.1 | − 2 | |
S1nv10 | (ANANARAR)2 | − 1.1 | + 4 | |
S1nv11 | (ANANARAR)3 | − 1.1 | + 6 | |
S1nv12 | (ANANARAR)4 | − 1.1 | + 8 | |
S1nv13 | (ANANARAR)5 | − 1.1 | + 10 | |
S1nv14 | (ANANARAR)6 | − 1.1 | + 12 | |
S1nv15 | (ANANARAR)8 | − 1.1 | + 16 | |
S1nv16 | (ANANARAR)9 | − 1.1 | + 18 | |
S1nv17 | ANANARARANANAR | − 1.064 | + 3 |