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Table 1 The amino acid sequence of SAPs designed in this study

From: An efficient expression tag library based on self-assembling amphipathic peptides

Schemes SAPs Amino acid sequence GRAVYa Net charge
  S1 (AEAEAKAK)2 − 0.95 0
Hydrophobic residues S1hv1 (IEIEIKIK)2 0.4 0
S1hv2 (LELELKLK)2 0.05 0
S1hv3 (VEVEVKVK)2 0.25 0
S1hv4 (FEFEFKFK)2 − 0.45 0
S1hv5 (GEGEGKGK)2 − 2.05 0
S1hv6 (PEPEPKPK)2 − 2.65 0
Hydrophilic residues S1cv1 (ADADAHAH)2 − 0.78 0
S1cv2 (AEAEAHAH)2 − 0.78 0
S1cv3 (ADADAKAK)2 − 0.95 0
S1cv4 (AEAEARAR)2 − 1.1 0
S1cv5 (ADADARAR)2 − 1.1 0
Length S1lv1 AEAEAKAK − 0.95 0
  S1lv2 (AEAEAKAK)3 − 0.95 0
  S1lv3 (AEAEAKAK)4 − 0.95 0
  S1lv4 (AEAEAKAK)5 − 0.95 0
  S1lv5 (AEAEAKAK)6 − 0.95 0
  S1lv6 (AEAEAKAK)7 − 0.95 0
  S1lv7 (AEAEAKAK)8 − 0.95 0
  S1lv8 (AEAEAKAK)9 − 0.95 0
  S1lv9 (AEAEAKAK)10 − 0.95 0
Net charge S1nv1 (ANANARAR)10 − 1.1  + 20
  S1nv2 (ANANADAD)10 − 1.1 − 20
  S1nv3 (ANANADAD)8 − 1.1 − 16
  S1nv4 (ANANADAD)7 − 1.1 − 14
  S1nv5 (ANANADAD)6 − 1.1 − 12
  S1nv6 (ANANADAD)4 − 1.1 − 8
  S1nv7 (ANANADAD)3 − 1.1 − 6
  S1nv8 (ANANADAD)2 − 1.1 − 4
  S1nv9 ANANADAD − 1.1 − 2
  S1nv10 (ANANARAR)2 − 1.1  + 4
  S1nv11 (ANANARAR)3 − 1.1  + 6
  S1nv12 (ANANARAR)4 − 1.1  + 8
  S1nv13 (ANANARAR)5 − 1.1  + 10
  S1nv14 (ANANARAR)6 − 1.1  + 12
  S1nv15 (ANANARAR)8 − 1.1  + 16
  S1nv16 (ANANARAR)9 − 1.1  + 18
  S1nv17 ANANARARANANAR − 1.064  + 3
  1. aThe total average of hydrophobicity (GRAVY, https://web.expasy.org/) was used to indicate the hydrophobicity of SAPs