Enhancing surfactin production by using systematic CRISPRi repression to screen amino acid biosynthesis genes in Bacillus subtilis

Table 2 Plasmids used in this study

Plasmids	Characteristics	Source/references
pJMP1	Pxyl-dCaS9, ErmR	[37]
pJMP2	Pveg-sgRNA^RR1, CmR	[37]
pmmgA	pJMP2 derivate, Pveg-sgRNA^mmgA	This work
pyhfS	pJMP2 derivate, Pveg-sgRNA^yhfS	This work
pasnB	pJMP2 derivate, Pveg-sgRNA^asnB	This work
pasnH	pJMP2 derivate, Pveg-sgRNA^asnH	This work
pasnO	pJMP2 derivate, Pveg-sgRNA^asnO	This work
pnadB	pJMP2 derivate, Pveg-sgRNA^nadB	This work
pyhdR	pJMP2 derivate, Pveg-sgRNA^yhdR	This work
pracX	pJMP2 derivate, Pveg-sgRNA^racX	This work
ppyrB	pJMP2 derivate, Pveg-sgRNA^pyrB	This work
ppyrC	pJMP2 derivate, Pveg-sgRNA^pyrC	This work
ppurF	pJMP2 derivate, Pveg-sgRNA^purF	This work
pglnA	pJMP2 derivate, Pveg-sgRNA^glnA	This work
pyrpC	pJMP2 derivate, Pveg-sgRNA^yrpC	This work
pracE	pJMP2 derivate, Pveg-sgRNA^racE	This work
pglmS	pJMP2 derivate, Pveg-sgRNA^glmS	This work
pybcM	pJMP2 derivate, Pveg-sgRNA^ybcM	This work
pmurD	pJMP2 derivate, Pveg-sgRNA^murD	This work
pmurC	pJMP2 derivate, Pveg-sgRNA^murC	This work
pbkdAA	pJMP2 derivate, Pveg-sgRNA^bkdAA	This work
pbkdAB	pJMP2 derivate, Pveg-sgRNA^bkdAB	This work
pH1	pJMP2 derivate, Pveg-sgRNA^yrpC, Pveg-sgRNA^racE	This work
pH2	pJMP2 derivate, Pveg-sgRNA^yrpC, Pveg-sgRNA^murC	This work
pH3	pJMP2 derivate, Pveg-sgRNA^racE, Pveg-sgRNA^murC	This work
pH4	pJMP2 derivate, Pveg-sgRNA^yrpC, Pveg-sgRNA^racE, Pveg-sgRNA^murC	This work

ISSN: 1475-2859