Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents

Table 2 Enantioselective synthesis of (R)-PE by Spore_E228S-GDH in organic solvents

Solvent	Log P	Yield (%)^a	ee (%)	Reduction durations	Productivity (mM h⁻¹ g⁻¹)
PBS		93 (95)	> 99 (> 99)	4.5 h	429.9 (439.1)
Ethyl acetate	0.68	88 (43)	> 99 (70)	4.5 h (10 h)	406.8 (89.4)
Ethyl acetate (6)^b	0.68	72	> 99	27 h	554.7
DMSO	− 1.3	81 (46)	> 99 (85)	4 h (10 h)	421.2 (95.7)
DMSO (3)	− 1.3	70	> 99	12 h	121.3
2-Propanol	0.33	85	98	5 h	353.6
2-Propanol (5)	0.33	70	95	25 h	291.2
Tert-butanol	0.6	72 (41)	96 (82)	5 h (12 h)	299.5 (71.1)
Methylbenzene	2.5	70	98	5 h	291.2
Cyclohexane	3.2	51 (39)	98 (92)	5 h (12 h)	212.2 (67.6)
Nonane	2.9	72	96	5 h	299.5
Hexane	3.5	80	98	4.5 h	369.8
Hexane (3)	3.5	70	93	13.5 h	107.9
Ethyl isovalerate	2.3	68	96	4.5 h	314.3
Urea	− 2.1	82	97	4.5 h	379.0
Urea (4)	− 2.1	70	93	18 h	80.9

Unless otherwise stated, all reactions were performed at 30 °C in 100 mM PBS buffer solution (pH 7.4) by using 0.1 g Spore_E228S-GDH. The reaction solution (2 mL) consists of 1.7 mL 100 mM PBS buffer (pH 7.4) and 0.3 mL solvents. AP concentration was 25 g/L (≈ 208 mM)
^a(R)-PE synthesis results with free enzymes in 200 mL PBS buffer (100 mM, pH 7.4) are given in parentheses. AP concentration was 8 g/L (≈ 67 mM)
^bThe values in parentheses in this column denote the recycled times for enantioselective synthesis

ISSN: 1475-2859