Fig. 1

Phenotypic characteristics of SS-II. a Cell line of T. reesei hyper-cellulolytic mutant SS-II. UV, ultraviolet; NTG, N-nitrosoguanidine. Biomass dry weight of T. reesei strains were measured in MA medium containing 2% (w/v) glucose (b), 2% (w/v) lactose (c), or 2% (w/v) Avicel (D) as the sole carbon source. FPase (e), CMCase (f), pNPCase (g), and pNPGase (h) activities and total secreted protein (i) of T. reesei strains were measured using Avicel as the carbon source. j Hydrolysis of pretreated corn stover using the crude enzyme from T. reesei strains at 20 FPU/g dry biomass. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test)