Enhanced production of styrene by engineered Escherichia coli and in situ product recovery (ISPR) with an organic solvent

Table 2 Bacterial strains and plasmids used in this study

	Description	References
Strain
XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI^qZ ΔM15 Tn10 (Tet^r)]	Stratagene^a
W3110	F⁻ l⁻ rph-1 INV(rrnD, rrnE)	Lab stock
YHP05	W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA ΔpykA	[12]
Plasmids
pTac15k	Km^R, p15A origin, P_tac promoter, 4.0 kb	[13]
pTrc99A	Amp^R, pBR322 origin, P_trc promoter, 4.2 kb	Pharmcia^b
pYHP	pTac15k, P_tac–aroG8/15–ydiB–aroK–pheA^{fbr, dm,} SacII region modification, P_pc113-glk-T_lpp, P_pc113-galP-T_lpp	[12]
pYHP-FDC	pYHP derivative, P_{BBa_J23100}-ScFDC (N-term 6×His-tag)	This study
pHB-CA	pTrc99A derivative, P_trc–SmPAL (C-term FLAG-tag)	[12]
pHB-CA-FDC	pTrc99A derivative, P_trc-SmPAL (C-term FLAG-tag)-ScFDC (N-term 6×His-tag)	This study

ISSN: 1475-2859