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Table 2 Bacterial strains and plasmids used in this study

From: Enhanced production of styrene by engineered Escherichia coli and in situ product recovery (ISPR) with an organic solvent

  Description References
Strain
 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZ ΔM15 Tn10 (Tetr)] Stratagenea
 W3110 F l rph-1 INV(rrnD, rrnE) Lab stock
 YHP05 W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA ΔpykA [12]
Plasmids
 pTac15k KmR, p15A origin, Ptac promoter, 4.0 kb [13]
 pTrc99A AmpR, pBR322 origin, Ptrc promoter, 4.2 kb Pharmciab
 pYHP pTac15k, Ptac–aroG8/15–ydiB–aroK–pheAfbr, dm, SacII region modification, Ppc113-glk-Tlpp, Ppc113-galP-Tlpp [12]
 pYHP-FDC pYHP derivative, PBBa_J23100-ScFDC (N-term 6×His-tag) This study
 pHB-CA pTrc99A derivative, Ptrc–SmPAL (C-term FLAG-tag) [12]
 pHB-CA-FDC pTrc99A derivative, Ptrc-SmPAL (C-term FLAG-tag)-ScFDC (N-term 6×His-tag) This study
  1. aStratagene Cloning Systems, La Jolla, CA, USA
  2. bPharmacia biotech, Uppsala, Sweden