Table 2 Bacterial strains and plasmids used in this study

Strain

 XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI^qZ ΔM15 Tn10 (Tet^r)]

Stratagene^a

 W3110

F⁻ l⁻ rph-1 INV(rrnD, rrnE)

Lab stock

 YHP05

W3110 Δcrr ΔtyrR ΔtrpE ΔtyrA ΔpykA

[12]

Plasmids

 pTac15k

Km^R, p15A origin, P_tac promoter, 4.0 kb

[13]

 pTrc99A

Amp^R, pBR322 origin, P_trc promoter, 4.2 kb

Pharmcia^b

 pYHP

pTac15k, P_tac–aroG8/15–ydiB–aroK–pheA^{fbr, dm,} SacII region modification, P_pc113-glk-T_lpp, P_pc113-galP-T_lpp

[12]

 pYHP-FDC

pYHP derivative, P_{BBa_J23100}-ScFDC (N-term 6×His-tag)

This study

 pHB-CA

pTrc99A derivative, P_trc–SmPAL (C-term FLAG-tag)

[12]

 pHB-CA-FDC

pTrc99A derivative, P_trc-SmPAL (C-term FLAG-tag)-ScFDC (N-term 6×His-tag)

This study