Table 2 Oligonucleotide primers used in this study
Name | Sequence (5′ → 3′) |
|---|---|
a) PCR primers: | |
RfpF_NdeI | ATATTCATATGGTTTCCAAGGGCGAGGAGG |
RfpR_NotI | TTTATAGCGGCCGCAAAAACAGCCGTTGCCAGAAAG |
014_cfp_F | ACCCTCCCTCGGCTTGTGCCGCGGCCGCAAAAGGAAAAGA |
014_cfp_RBS2_R | GGAATATAGCAAATAAGGAGGAGGAAAATGAGCAAGGGCGAGGAGCTG |
014_pib_F | AGCTTTTGTTCGGATCCAGCAAACTGCAAAAAAAAGTCCGCTGA |
014_pib_RBS2_R | TTTCCTCCTCCTTATTTGCTATATTCCACCTGAATGGGTTGCGAATCGCGTTTAG |
YfpF_NdeI | CGTGCATATGAGCAAAGGTGAAGAAC |
YfpR_NotI | TGCTAGCGGCCGCAGGACAGCTATTGTAGATAAG |
032_002_F | CCCGGTTTGATAGGGATAAG |
032_002_R | TACGGATGAGCATTCATCAG |
032_014_F | CTGATGAATGCTCATCCGTAGATCCAGCAAACTGCAAAAAAAAG |
032_014_R | CAATAGCTGTCCTGCGGCCGATCGAGTTGCTGGAGATTGTG |
032_017_F | CGGCCGCAGGACAGCTATTGTA |
032_017_R | CTTATCCCTATCAAACCGGGATACCCTCCCTCGGCTTGTG |
rpsJ_yfpF_SnaBI | GAATGCTCATCCGTAATTACCGCGAAACGGATACCCTCCCTC |
rpsJ_yfpR_NheI | CTTATCCCTATCAAACCGGGGGCCTTTAGGTCTTCTTCTG |
pibfxs_cfpF_SnaBI | CTTATCCCTATCAAACCGGGATCGAGTTGCTGGAGATTGT |
pibfxs_cfpR_NheI | GAATGCTCATCCGTAATTACGGCCTTTAGGTCTTCTTCTG |
021_rpsJ_F | AGCTTGACCTGTGAAGTGAA |
021_rpsJ_R | CTGAGATCAGCTTTTGTTCG |
021_ibpfxs_F | CGAACAAAAGCTGATCTCAGAGGCCTTTAGGTCTTCTTCT |
021_ibpfxs_R | TTCACTTCACAGGTCAAGCTAAGGAAAAGATCCGGCAAAC |
b) qPCR primers: | |
cysG_F1 | GTATTCCACTCACGCATCGC |
cysG_R1 | CGCCACCGGTTTTTAAGTGT |
rplC_F2 | TGACCGATAGAACCCGGAAC |
rplC_R2 | CTGGAACTTCCGTACCCAGG |